TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Infestans
         (633 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738895.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   270   4e-72

>gb|GG738895.1| Naegleria gruberi genomic scaffold NAEGRscaffold_51, whole genome
             shotgun sequence
          Length = 333733

 Score =  270 bits (689), Expect = 4e-72
 Identities = 202/593 (34%), Positives = 295/593 (49%), Gaps = 43/593 (7%)
 Frame = +1

Query: 84    SKSQDE-GVNGEGDDL------------QPIETLVPILAGNTLLPGAETAAQCYCGHQFG 130
             S+S DE GVNG  D +              ++  V IL+G  L+   +  A CY G QFG
Sbjct: 45526 SESGDERGVNGVNDLITVEHLMKQQEKEHDLDNFVNILSGYDLVNSTKYYAHCYGGFQFG 45705

Query: 131   FFSGQLGDGAALYLGEV-----------------------VAVDERWELQLKGSGLTPYS 167
              ++GQLGDG A+ +G+V                       +     WELQ KG+G TP+S
Sbjct: 45706 NWAGQLGDGRAISMGQVETPFTDMDSSGFEFNNSRNSYNYIKPKRLWELQFKGAGHTPFS 45885

Query: 168   RTADGRKVLRSTLREFLCSENMHALGVPTTRAGSVVTSKETQVLRDIFYNGDAKMEPTAV 227
             R ADGR VLRS++REFL SE M +LG+ TTRA S+V SKE  VLRD FY+ + K E  A+
Sbjct: 45886 RHADGRAVLRSSIREFLGSEFMDSLGIATTRAFSLVRSKEKAVLRDEFYDNNPKYEYGAI 46065

Query: 228   VTRIAKSFLRFGSFEIFKDEDKLTGLAGPSAHLENKEEMMREMLDFTIRQYYSE--ISG- 284
             V R+A +F+RFGSF+IF    +   +      LE K+  +  +  + I+ ++    I+G 
Sbjct: 46066 VLRVAPTFVRFGSFDIF--NYRYHPINEKEKALEEKKN-IEVLARYVIKNHFPHLWINGD 46236

Query: 285   --ARKYEKFFQEVVRRTAMLVAKWQSIGFCHGVLNTDNMSIVGDTLDYGPFGFMEHFDPK 342
                   EKF +E+VRRTA L A W S+GF HGVLNTDNMSI+G T+DYGPFGF+++F   
Sbjct: 46237 LTLELKEKFSKEIVRRTAKLCADWMSVGFVHGVLNTDNMSILGLTIDYGPFGFVDYFSED 46416

Query: 343   HICNTSDDRGRYRYEAQPEVCKWNCGVL--ADQLGLVTERAGLEPILESFDAVYEAEYMR 400
              + N SD  GRYRY+ QP +  WN   L  A    L+ E      +L  +   +E  Y+ 
Sbjct: 46417 FVPNNSDSDGRYRYKNQPAIVFWNLQKLMRAFTPTLLPEEY-FAKVLNVYAPHFEHYYLM 46593

Query: 401   LMREKLGLSDEEKEDKMLVDTLFDVLAFTGADFTCTFRYLSELDVFETGDCREQVLNKLV 460
               R+KLGL         ++DT  DV  F   D         + +     D  E +   L 
Sbjct: 46594 NFRKKLGLI----SSSTIIDTSEDVTNFDMFD--------GDSENLRNEDW-ELIEGFLA 46734

Query: 461   AVSETLAQQKRKLELDSGGVSDAQFDMVVMLLQENPVRARQYGITPALVAQIKANREAKK 520
              ++E  A       L S     A+   V   L +N ++ R +                  
Sbjct: 46735 WMNENRADFTNFFRLLSNVKKGAE---VSQELLDNLLQTRMHA----------------- 46854

Query: 521   LLDATTDEERMDSIRTVWVDWIDVYISRVKEQGDAASDADRRRRMLDVNPLFVLRNHVAQ 580
               D T  E  +  ++    +W+ +Y  R+  +    SD +R+ +M   NP ++LRN++AQ
Sbjct: 46855 --DHTPSETTVSELK----NWLSIYTKRL--ESVPLSDEERKTQMDKTNPRYILRNYIAQ 47010

Query: 581   KAIDFAHEGDYDAVQHIFELVTNPFDEPTDDRDLEYARPQDSSTAPLCVSXSS 633
             K I  A E DY  +   + ++ NP+D  + + + ++      S+  L +S SS
Sbjct: 47011 KVIKSAEEFDYGPLYEYYNVLRNPYDNHSTEFEEKFGGNAPLSSRCLKLSCSS 47169


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.318    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,381,515
Number of Sequences: 784
Number of extensions: 319402
Number of successful extensions: 1247
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 21
Number of HSP's that attempted gapping in prelim test: 1134
Number of HSP's gapped (non-prelim): 305
length of query: 633
length of database: 13,654,695
effective HSP length: 108
effective length of query: 525
effective length of database: 13,570,023
effective search space: 7124262075
effective search space used: 7124262075
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 117 (49.7 bits)